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cytomegalovirus cmv promoter  (OriGene)


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    Structured Review

    OriGene cytomegalovirus cmv promoter
    Cytomegalovirus Cmv Promoter, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cmv+promoter/MIR1255a+Human+MicroRNA+Expression+Plasmid/pm42218122-64-9-15
    Average 94 stars, based on 1 article reviews
    cytomegalovirus cmv promoter - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: StarD5 Plays a Critical Role in the Hepatocyte ER Stress Survival Response.
    Article Snippet: .. Ad-CMV-hStarD5.MycDDK and Ad-CMV-β-gal were prepared by SignaGen Laboratories (Frederick, MD, USA) using a plasmid encoding human StarD5 cDNA fused to myc and flag tags, driven by the CMV promoter (Catalog # RC202407), which was from Origene (Rockville, MD, USA). .. Multiplicity of infection (MOI) was quantified with the Adeno-X Rapid Test Kit from Takara-Bio (Cat#632270, San Jose, CA, USA).

    Article Title: Vector with an endothelial-specific promoter for treating factor V, VII, VIII, or IX deficiency
    Article Snippet: Luciferase Assay The Stab2.2 and Stab2.3 promoter sequences were cloned into the pNL1.1 [NanoLuc®] plasmid (Promega) using XhoI and HindIII restriction sites. .. Plasmid encoding the endothelial transcription factor Ets-1 (NM_001143820) under the control of CMV promoter was bought from Origene. .. Stab2.2 or Stab2.3 luciferase expressing vectors were transiently transfected using LipofectamineTM 2000 Transfection Reagent (Thermo Fisher scientific) in 293T cell line either alone or in combination with Ets-1 transcription factor.

    Article Title: Occludin Is Essential to Maintain Normal Alveolar Barrier Integrity and Its Protective Role During ARDS Progression
    Article Snippet: .. The plasmid pcDNA3.1 was from Invitrogen/Thermofisher (Carlsbad, CA, USA, catalog #V79020). pCMV-occludin expresses a GFP-tagged mouse occludin from the CMV promoter (Origene, Rockville, MD, USA, catalog # MG226013). pEGFP-C1 expresses GFP from the CMV promoter (Clontech, Mountain View, CA, USA, catalog # 6084-1). .. Four distinct RFP-expressing occludin shRNA plasmids (catalog # TF501526) and a scrambled shRNA plasmid (catalog # TR30015) were purchased from Origene (Rockville, MD, USA).

    Article Title: RORγ Bridges Cancer-Driven Lipid Dysmetabolism and Myeloid Immunosuppression
    Article Snippet: .. A plasmid vector constitutively expressing the Rorc gene under the control of the CMV promoter (pCMV6- Rorc ; OriGene, No. MR222309) was cotransfected with a plasmid expressing the luciferase gene ( Luc ) under the control of the minimal promoter of the Il17a gene (minIL17prom-Luc; Addgene, No. 20124; RRID: Addgene_20124). ..

    Expressing:

    Article Title: The Welander TIA1 mutation dedifferentiates insulin-producing cells: Reversal by a GLP-1 receptor agonist
    Article Snippet: .. Lentiviral particles expressing GFP under the CMV promoter (pLenti-mGFP-Puro) were obtained from OriGene Technologies, Inc, with a scramble or an shRNA sequence targeting human MYC (CAT#: RC201611L4V). ..

    Article Title: ZNF671 Silencing Affects Signaling Pathways in Head and Neck Cancer via Activation of Oncogenic Non-Coding RNAs
    Article Snippet: .. In order to stably overexpress ZNF671 in UM-SCC-1 cells, we utilized the pLenti-C-Myc-DDKP2A-Puro construct vector expressing the ZNF671 gene as a Flag-tagged fusion protein under the control of the CMV promoter (Cat#RC206413L3, Origene, Rockville, MD, USA). .. We utilized the empty pLenti-C-Myc-DDK-P2A-Puro vector as a negative control (Cat# PS100092, Origene).

    Article Title: RORγ Bridges Cancer-Driven Lipid Dysmetabolism and Myeloid Immunosuppression
    Article Snippet: .. A plasmid vector constitutively expressing the Rorc gene under the control of the CMV promoter (pCMV6- Rorc ; OriGene, No. MR222309) was cotransfected with a plasmid expressing the luciferase gene ( Luc ) under the control of the minimal promoter of the Il17a gene (minIL17prom-Luc; Addgene, No. 20124; RRID: Addgene_20124). ..

    shRNA:

    Article Title: The Welander TIA1 mutation dedifferentiates insulin-producing cells: Reversal by a GLP-1 receptor agonist
    Article Snippet: .. Lentiviral particles expressing GFP under the CMV promoter (pLenti-mGFP-Puro) were obtained from OriGene Technologies, Inc, with a scramble or an shRNA sequence targeting human MYC (CAT#: RC201611L4V). ..

    Sequencing:

    Article Title: The Welander TIA1 mutation dedifferentiates insulin-producing cells: Reversal by a GLP-1 receptor agonist
    Article Snippet: .. Lentiviral particles expressing GFP under the CMV promoter (pLenti-mGFP-Puro) were obtained from OriGene Technologies, Inc, with a scramble or an shRNA sequence targeting human MYC (CAT#: RC201611L4V). ..

    Control:

    Article Title: Vector with an endothelial-specific promoter for treating factor V, VII, VIII, or IX deficiency
    Article Snippet: Luciferase Assay The Stab2.2 and Stab2.3 promoter sequences were cloned into the pNL1.1 [NanoLuc®] plasmid (Promega) using XhoI and HindIII restriction sites. .. Plasmid encoding the endothelial transcription factor Ets-1 (NM_001143820) under the control of CMV promoter was bought from Origene. .. Stab2.2 or Stab2.3 luciferase expressing vectors were transiently transfected using LipofectamineTM 2000 Transfection Reagent (Thermo Fisher scientific) in 293T cell line either alone or in combination with Ets-1 transcription factor.

    Article Title: Squamous cell carcinoma antigen-1/SerpinB3 is an endogenous skin injury response element
    Article Snippet: .. The day after seeding, cells were transfected with pCMV6-Entry containing a c-terminal Myc-DDK tag under control of a CMV promoter (Origene #RC202683) using Attractene reagent (Qiagen) according to manufacturer’s procedure and incubated for 24 hours prior to immunofluorescence. ..

    Article Title: ZNF671 Silencing Affects Signaling Pathways in Head and Neck Cancer via Activation of Oncogenic Non-Coding RNAs
    Article Snippet: .. In order to stably overexpress ZNF671 in UM-SCC-1 cells, we utilized the pLenti-C-Myc-DDKP2A-Puro construct vector expressing the ZNF671 gene as a Flag-tagged fusion protein under the control of the CMV promoter (Cat#RC206413L3, Origene, Rockville, MD, USA). .. We utilized the empty pLenti-C-Myc-DDK-P2A-Puro vector as a negative control (Cat# PS100092, Origene).

    Article Title: Modulating the serine metabolism in human differentiated astrocytes: an integrated multi omics approach
    Article Snippet: When the cells reached 90%–95% confluency (approximately every 4-5 days), they were re-seeded to the initial density (25000 cells/cm 2 ) as single cells in astrocyte medium, and cultured on Geltrex, following 5–10 min incubation with accutase (Thermo Fisher A1110501), pipetting and washing with DMEM. .. Lentiviral vectors to obtain overexpression of GFP-tagged PHGDH, PSAT and PSP (together with the empty GFP vector) under a CMV promoter were purchased from Origene (Catalog numbers RC203949L4 for PHGDH, RC202475L4 for PSAT, RC209090L4 for PSP and PS100093 as control). ..

    Article Title: RORγ Bridges Cancer-Driven Lipid Dysmetabolism and Myeloid Immunosuppression
    Article Snippet: .. A plasmid vector constitutively expressing the Rorc gene under the control of the CMV promoter (pCMV6- Rorc ; OriGene, No. MR222309) was cotransfected with a plasmid expressing the luciferase gene ( Luc ) under the control of the minimal promoter of the Il17a gene (minIL17prom-Luc; Addgene, No. 20124; RRID: Addgene_20124). ..

    Transfection:

    Article Title: Squamous cell carcinoma antigen-1/SerpinB3 is an endogenous skin injury response element
    Article Snippet: .. The day after seeding, cells were transfected with pCMV6-Entry containing a c-terminal Myc-DDK tag under control of a CMV promoter (Origene #RC202683) using Attractene reagent (Qiagen) according to manufacturer’s procedure and incubated for 24 hours prior to immunofluorescence. ..

    Incubation:

    Article Title: Squamous cell carcinoma antigen-1/SerpinB3 is an endogenous skin injury response element
    Article Snippet: .. The day after seeding, cells were transfected with pCMV6-Entry containing a c-terminal Myc-DDK tag under control of a CMV promoter (Origene #RC202683) using Attractene reagent (Qiagen) according to manufacturer’s procedure and incubated for 24 hours prior to immunofluorescence. ..

    Immunofluorescence:

    Article Title: Squamous cell carcinoma antigen-1/SerpinB3 is an endogenous skin injury response element
    Article Snippet: .. The day after seeding, cells were transfected with pCMV6-Entry containing a c-terminal Myc-DDK tag under control of a CMV promoter (Origene #RC202683) using Attractene reagent (Qiagen) according to manufacturer’s procedure and incubated for 24 hours prior to immunofluorescence. ..

    Stable Transfection:

    Article Title: ZNF671 Silencing Affects Signaling Pathways in Head and Neck Cancer via Activation of Oncogenic Non-Coding RNAs
    Article Snippet: .. In order to stably overexpress ZNF671 in UM-SCC-1 cells, we utilized the pLenti-C-Myc-DDKP2A-Puro construct vector expressing the ZNF671 gene as a Flag-tagged fusion protein under the control of the CMV promoter (Cat#RC206413L3, Origene, Rockville, MD, USA). .. We utilized the empty pLenti-C-Myc-DDK-P2A-Puro vector as a negative control (Cat# PS100092, Origene).

    Construct:

    Article Title: ZNF671 Silencing Affects Signaling Pathways in Head and Neck Cancer via Activation of Oncogenic Non-Coding RNAs
    Article Snippet: .. In order to stably overexpress ZNF671 in UM-SCC-1 cells, we utilized the pLenti-C-Myc-DDKP2A-Puro construct vector expressing the ZNF671 gene as a Flag-tagged fusion protein under the control of the CMV promoter (Cat#RC206413L3, Origene, Rockville, MD, USA). .. We utilized the empty pLenti-C-Myc-DDK-P2A-Puro vector as a negative control (Cat# PS100092, Origene).

    Over Expression:

    Article Title: Modulating the serine metabolism in human differentiated astrocytes: an integrated multi omics approach
    Article Snippet: When the cells reached 90%–95% confluency (approximately every 4-5 days), they were re-seeded to the initial density (25000 cells/cm 2 ) as single cells in astrocyte medium, and cultured on Geltrex, following 5–10 min incubation with accutase (Thermo Fisher A1110501), pipetting and washing with DMEM. .. Lentiviral vectors to obtain overexpression of GFP-tagged PHGDH, PSAT and PSP (together with the empty GFP vector) under a CMV promoter were purchased from Origene (Catalog numbers RC203949L4 for PHGDH, RC202475L4 for PSAT, RC209090L4 for PSP and PS100093 as control). ..

    Luciferase:

    Article Title: RORγ Bridges Cancer-Driven Lipid Dysmetabolism and Myeloid Immunosuppression
    Article Snippet: .. A plasmid vector constitutively expressing the Rorc gene under the control of the CMV promoter (pCMV6- Rorc ; OriGene, No. MR222309) was cotransfected with a plasmid expressing the luciferase gene ( Luc ) under the control of the minimal promoter of the Il17a gene (minIL17prom-Luc; Addgene, No. 20124; RRID: Addgene_20124). ..



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    Image Search Results


    Evaluation of dCas13e to modulate splicing defects of ABCA4 c.5461-10T>C in ARPE19 (A) A schematic illustration of the pSPL3– ABCA4 c.5461-10T>C minigene and the all-in-one construct of a U6 promoter-driven sgRNA sequence followed by the CMV promoter-driven RBFOX1N-dCas13e-C factor. The sgRNA targeted the same region downstream of E39 (green) as a previously reported antisense oligonucleotide (AON) target site. Positions of SD6 and SA2 primers for splicing product amplification are displayed. (B) RT-PCR results of ABCA4 splice variants in ARPE19 cells transfected with the ABCA4 minigene system (MT), RBFOX1N-dCas13e-C, sgRNAs targeting mCherry (control), or ABCA4 . ACTB was used as a housekeeping gene. Mock control represents ARPE19 without transfection of the ABCA4 minigene system. (C) Gel quantification of relative transcript expression of ABCA4 splice variants compared to MT control without treatment. Data are represented as mean ± SEM ( n = 5). (D) Gel quantification of the ratio of disease transcript to FL transcript. Data are represented as mean ± SEM ( n = 3).

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: Using RNA-targeting CRISPR-Cas13 and engineered U1 systems to target ABCA4 splice variants in Stargardt disease

    doi: 10.1016/j.omtn.2025.102789

    Figure Lengend Snippet: Evaluation of dCas13e to modulate splicing defects of ABCA4 c.5461-10T>C in ARPE19 (A) A schematic illustration of the pSPL3– ABCA4 c.5461-10T>C minigene and the all-in-one construct of a U6 promoter-driven sgRNA sequence followed by the CMV promoter-driven RBFOX1N-dCas13e-C factor. The sgRNA targeted the same region downstream of E39 (green) as a previously reported antisense oligonucleotide (AON) target site. Positions of SD6 and SA2 primers for splicing product amplification are displayed. (B) RT-PCR results of ABCA4 splice variants in ARPE19 cells transfected with the ABCA4 minigene system (MT), RBFOX1N-dCas13e-C, sgRNAs targeting mCherry (control), or ABCA4 . ACTB was used as a housekeeping gene. Mock control represents ARPE19 without transfection of the ABCA4 minigene system. (C) Gel quantification of relative transcript expression of ABCA4 splice variants compared to MT control without treatment. Data are represented as mean ± SEM ( n = 5). (D) Gel quantification of the ratio of disease transcript to FL transcript. Data are represented as mean ± SEM ( n = 3).

    Article Snippet: For lentiviral transduction efficiency testing, ARPE19 was transduced using with lentiviruses with dsRed reporter driven by CMV promoter (Addgene #141148) and the fluorescence signal was analyzed after 4 days using MOI of 30.

    Techniques: Construct, Sequencing, Amplification, Reverse Transcription Polymerase Chain Reaction, Transfection, Control, Expressing